a549 luc cells  (ATCC)

Journal: International Journal of Biological Sciences

Article Title: Ugonin P mitigates osteolytic bone metastasis by suppressing MDK via upregulating miR-223-3p expression

doi: 10.7150/ijbs.111356

Figure Lengend Snippet: Ugonin P inhibits osteolytic bone metastasis in vivo . (A) Intra tibial injection of A549 and MDA-MB-231 cells into nude mice followed by intraperitoneally treated with Ugonin P (15 mg/kg) for alternative days for 4 weeks. (B-C) Representative IVIS images of bone metastasis at 1 and 4 weeks. (D-G) Quantification of emitted photons from each tumor by using IVIS and Mouse body weight was monitored three times a week over the four weeks. (H) After four weeks of Ugonin P treatment, all mice were humanely euthanized. The dissected legs from both the control and Ugonin P-treated groups were evaluated for tumor luminescent intensity to assess the treatment's effects. n= 4 mice per group.

Article Snippet: A549 and MDA-MB-231 Luc cells (1 × 105) were injected into the right tibia of 4-week-old nude mice obtained from BioLASCO Taiwan Co., Ltd. (Taipei, Taiwan).

Techniques: In Vivo, Injection, Control

Journal: Respiratory Research

Article Title: Aggregatibacter is inversely associated with inflammatory mediators in sputa of patients with chronic airway diseases and reduces inflammation in vitro

doi: 10.1186/s12931-024-02983-z

Figure Lengend Snippet: Determination of the minimal effective MOI (mMOI) of a ) A. actinomycetemcomitans and b ) A. aphrophilus based on NF-κB pathway activation and determination of the mMOI of c ) A. actinomycetemcomitans and d ) A. aphrophilus based on IL-8 production in 3-D lung epithelial cells. On the vertical axis % NF-kB pathway activation / IL-8 production compared to LPS-stimulated cells is shown, on the horizontal axis the tested MOI is depicted. The mMOI was defined as the lowest MOI with anti-inflammatory activity (i.e. < 50% NF-kB pathway activation / IL-8 production compared to LPS-stimulated cells as visualized by a dotted line, p < 0,05). e ) Western blot analysis of proteins (i.e. IκBα, p65 and phosphorylated (p)-IκBα) produced by 3-D A549 cells stimulated with LPS in the presence or absence of A. actinomycetemcomitans (targeted MOI 50:1) for 4 h (representative replicate is shown, image contains cropped blot – the full original blot is shown in Supplementary file_Western blot image). f ) Band intensity (normalised to β-actin) of Western blot at 4 h of 3-D A549 cells stimulated with LPS with/without A. actinomycetemcomitans . Results are expressed as a percentage of the positive control (i.e. LPS). NC: negative control (untreated 3-D A549 cells in serum-free GTSF-2 medium), A.ac. A. actinomycetemcomitans . Data represent mean ± SEM, * p < 0.05, n ≥ 3

Article Snippet: A previously developed organotypic 3-D lung cell culture model of the A549 alveolar epithelial cell line (ATCC CCL185) or the NF-κB–luciferase-transfected A549 recombinant stable cell line (BPS Bioscience, San Diego, CA, US) was used that exhibits in vivo-like phenotypic and functional properties of alveolar epithelial cells, including barrier function (localized expression of tight junctional markers), apical and basolateral polarity, and responds to infection in ways that are relevant to the infection process in vivo, including cytokine secretion [ , – ].

Techniques: Activation Assay, Activity Assay, Western Blot, Produced, Positive Control, Negative Control